Hemocytometer Cell Counting Calculator
Determine cell density and total yield from grid counts. Ideal for mammalian cell culture, yeast suspensions, and general micro-organism counts.
Grid Counts & Factors
Input your counting counts, grid squares, and dilution factor.
Cell Concentration
* Equal to 500,000 cells/mL.
Expert Tip
When counting, implement the "border rule" consistently: count cells touching the top and left borders of a square, but exclude cells touching the bottom and right borders.
Methodology & Grid Calculations
Hemocytometer Formula
Under a standard Neubauer chamber (depth 0.1 mm), each large corner square holds exactly 0.1 mm³ or 10⁻⁴ mL of fluid. Therefore, concentration is:
Neubauer Grid Dimensions
A standard hemocytometer contains 9 large squares (1 mm x 1 mm each):
- Large Square Area: 1.0 mm²
- Chamber Depth: 0.1 mm
- Volume per Square: 0.1 mm³ (10⁻⁴ mL)
How to Calculate Cell Concentration with a Hemocytometer
Hemocytometers are standard grids used to count suspended cells under a microscope:
Count Cells in Grid Squares
Count total cells in the large outer corner squares (each represents a volume of 0.1 mm^3 or 10^-4 mL).
Calculate Average Cell Count
Divide total counted cells by the number of large squares counted.
Incorporate Dilution
Multiply the average by the dilution factor and by the volume factor of 10,000: Cells/mL = (Total Cells / Squares) × Dilution Factor × 10,000.