Protein Solubility Calculator
Predict protein solubility patterns using either the Cohn salting-out model or pH-dependent isoelectric precipitation curves.
Input Parameters
Enter details for calculation.
Estimated Protein Solubility
Expert Tip
Salting-out is common for purification. At low salt concentrations (salting-in), solubility increases; at high ionic strength (salting-out), water molecules are sequestered, causing proteins to aggregate.
Calculation Methodology & Details
Formula
Cohn model describes salting-out. The pH model approximates isoelectric precipitation: solubility decreases parabolically as pH approaches the isoelectric point.
Important Disclaimer
The calculations provided are for educational and general planning guidelines only. They do not replace professional laboratory or environmental analysis or certified scientific advice.
How to Predict Solubility
Steps to estimate protein solubility:
Select Model
Choose between Cohn salting-out (salt-dependent) or isoelectric precipitation (pH-dependent).
Set constants
Provide reference solubility at zero salt (β) or minimum solubility at pI.
Observe output
Vary salt or pH to calculate the estimated solubility in mg/mL.