MOI Calculator
Calculate the Multiplicity of Infection (MOI) or determine the required volume of viral stock needed for a target cell count.
Input Parameters
Enter details for calculation.
Actual MOI
- Uninfected cells: 36.8%
- Cells with 1 virus: 36.8%
- Multiple infections: 26.4%
Expert Tip
MOI represents the ratio of viral particles to target cells. Actual infection rate follows a Poisson distribution: at MOI = 1, ~36.8% of cells remain uninfected.
Calculation Methodology & Details
Formula
For Poisson probability P(k) = (e^(-MOI) * MOI^k) / k! where k represents the number of viruses infective on a single cell.
Important Disclaimer
The calculations provided are for educational and general planning guidelines only. They do not replace professional laboratory or environmental analysis or certified scientific advice.
How to Calculate MOI
Steps to compute MOI or required stock viral volumes:
Choose Mode
Select whether to calculate actual MOI or required virus stock volume.
Enter counts
Input cell concentration, suspension volume, and viral stock titer.
Evaluate Poisson
Analyze the percentage of cells that will receive zero, one, or multiple viral particles.