Lineweaver-Burk Plot Calculator
Compute Michaelis-Menten kinetic constants (Km and Vmax) from substrate-velocity data points using a double-reciprocal linear regression model.
Input Parameters
Enter details for calculation.
Maximum Velocity (V_max)
Michaelis Constant (K_m)
Fit Quality (R²)
Expert Tip
A double-reciprocal (1/[S] vs 1/v) plot linearizes the Michaelis-Menten curve. Ensure to check the R² value to evaluate the goodness of fit.
Calculation Methodology & Details
Formula
Calculates the linear regression on the reciprocals of substrate [S] and velocity v. Km and Vmax are solved from the slope (m) and y-intercept (c).
Important Disclaimer
The calculations provided are for educational and general planning guidelines only. They do not replace professional laboratory or environmental analysis or certified scientific advice.
How to use Lineweaver-Burk fit
Steps to linearize enzyme kinetics data:
Input Data Points
Enter substrate concentration [S] and velocity v for at least 3 distinct experiments.
Analyze Regression
The calculator plots reciprocal values and performs linear regression.
Interpret Km and Vmax
Read Vmax from the y-intercept reciprocal, and Km from the slope.